mARG1 cassette 2

LOCUS       mARG1 cassette 2              10546 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac 
            trasposon plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    mARG1 cassette 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG
  TITLE     Ultrasound imaging of gene expression in mammalian cells.
  JOURNAL   Science. 2019 Sep 27;365(6460):1469-1475. doi: 
            10.1126/science.aax4804.
  PUBMED    31604277
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   91..153
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        587..936
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    595..613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    631..649
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    667..685
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    703..721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    739..757
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    775..793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    811..829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     875..899
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1898..1954
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      2081..2090
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      2085..2094
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2738..2794
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3077..3133
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3959..4015
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4319..4375
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4676..4732
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5051..5107
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5540..5596
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5903..5922)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    6333..6454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6370..6389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6424..6443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7217..7251
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7742..7758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7742..7758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7755..7777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7766..7782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7790..7820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7835..7856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7973..7990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8144..8732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8224..8243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8903..9763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9526..9545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9764..9868)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9894..10349)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10031..10052)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10243..10262
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10476..10498
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10490..10507
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10491..10507
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc
       61 gacacgctgc agaacacgca gctagattaa ccctagaaag ataatcatat tgtgacgtac
      121 gttaaagata atcatgtgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt
      181 tatttattaa tttgaataga tattaagttt tattatattt acacttacat actaataata
      241 aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaacaaa aactcaaaat
      301 ttcttctata aagtaacaaa acttttatga gggacagccc ccccccaaag cccccaggga
      361 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
      421 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
      481 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
      541 ctggggggat acggggaaaa ggcctccacg gccactagtt tcactcgagt ttactcccta
      601 tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga
      661 ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag
      721 aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta
      781 tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa cgtatgtcga
      841 ggtaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac cgtcagatcg
      901 cctggagcaa ttccacaaca cttttgtctt atacttggta cctatgcatg ccaccatgac
      961 cgtgctgacc gacaagcgga agaagggcag cggcgccttc atccaggacg acgagacaaa
     1021 agaggtgctg agcagagccc tgagctacct gaagtccggc tacagcatcc acttcaccgg
     1081 acctgccggc ggaggcaaga catctctggc tagagccctg gccaagaaac ggaagcggcc
     1141 cgtgatgctg atgcacggca accacgagct gaacaacaag gacctgatcg gcgatttcac
     1201 cggctacacc agcaaaaagg tgatcgacca gtacgtgcgg agcgtgtaca agaaagacga
     1261 acaggtgtcc gagaactggc aggacggcag actgctggaa gccgtgaaga atggctacac
     1321 cctgatctac gacgagttca ccagaagcaa gcccgctacc aacaacatct tcctgagcat
     1381 ccttgaggag ggcgtgctgc ccctgtacgg cgtgaagatg accgaccctt tcgtgcgcgt
     1441 gcaccccgac ttcagagtga tctttaccag caaccccgcc gagtatgccg gcgtgtacga
     1501 tacccaggac gccctgctgg accggctgat caccatgttc atcgactaca aggacatcga
     1561 ccgggaaacc gctatcctga ccgagaaaac tgacgtggaa gaagacgagg cccggaccat
     1621 cgtgaccctg gtggccaacg tgcggaacag aagcggcgac gagaatagca gcggcctgag
     1681 cctgagagcc agcctgatga ttgccaccct ggccacccag caggacatcc ctatcgatgg
     1741 cagcgacgag gacttccaga ccctgtgcat cgacatcctg caccaccccc tgaccaagtg
     1801 cctggacgaa gagaacgcca agagcaaggc cgagaagatc attctcgaag agtgcaagaa
     1861 catcgacacc gaggagaagg gtgccccggg atctggcgca acaaatttta gtcttttaaa
     1921 gcaggcagga gacgtcgagg aaaaccctgg acccgtgagc gagacaaacg agacaggcat
     1981 ctacatcttc agcgccatcc agacagacaa ggatgaggaa ttcggcgccg tggaagtgga
     2041 agggaccaag gctgagacat tcctgatccg gtataaggac gccgccatgg tggccgccga
     2101 agtgcccatg aagatctacc accccaaccg gcagaacctg ctgatgcacc agaatgccgt
     2161 ggccgccatc atggacaaga acgacaccgt gatccccatc agcttcggca acgtgttcaa
     2221 gagcaaagag gacgtgaagg tgctcctgga aaacctgtac ccccagttcg agaagctgtt
     2281 ccccgccatc aagggaaaga tcgaagtggg cctgaaggtg atcggcaaga aagagtggct
     2341 cgaaaagaaa gtgaacgaga accccgagct ggaaaaagtg tccgccagcg tgaagggcaa
     2401 gagcgaggcc gctggctact acgagagaat ccagctgggc ggcatggccc agaagatgtt
     2461 cacaagcctg cagaaagaag tgaaaaccga cgtgttcagc cccctggaag aagccgccga
     2521 ggccgccaaa gccaatgagc ctacaggcga aacaatgctg ctgaacgcca gcttcctgat
     2581 caacagagag gatgaggcca agttcgacga gaaagtcaat gaggcccacg agaactggaa
     2641 ggataaggcc gacttccact acagcggccc ctggcccgcc tacaacttcg tgaacatccg
     2701 gctgaaggtg gaagagaagg gggcacctgg ctcgggagcg accaacttct cattactcaa
     2761 acaagccgga gacgttgagg agaatccagg ccctgtgctg cacaagctcg tgaccgcccc
     2821 catcaacctg gtcgtgaaga tcggcgagaa ggtgcaggaa gaggccgaca agcagctgta
     2881 cgacctgccc accatccagc agaagctgat ccagctgcag atgatgttcg agctgggcga
     2941 gatccccgag gaagccttcc aggaaaaaga ggacgaactg ctgatgagat acgagatcgc
     3001 caagcggcgc gagattgagc agtgggaaga actgacccag aagcggaatg aggaaagcgg
     3061 tgccccggga tctggcgcaa caaattttag tcttttaaag caggcaggag acgtcgagga
     3121 aaaccctgga cccgtgggcg agctgctgta cctctacggc ctgatcccca ccaaagaggc
     3181 cgctgctatc gagcccttcc cattctacaa gggcttcgac ggcgagcaca gcctgtaccc
     3241 tatcgccttc gaccaagtga ccgccgtggt gttcaagctg gacgccgaca cctacagcga
     3301 gaaagtgatc caggaaaaga tggaacagga catgagctgg ctgcaggaaa aggccttcca
     3361 ccaccacgag acagtggccg ccctgtatga ggaattcacc atcatccccc tgaagttctg
     3421 caccatctat aagggagagg aatccctgca ggccgccatc gagatcaaca aagagaagat
     3481 cgaaaactcc ctgaccctgc tgcagggcaa cgaggaatgg aacgtgaaga tctactgcga
     3541 cgacaccgag ctgaagaagg gcatcagcga gacaaacgag agcgtgaagg ccaagaagca
     3601 ggaaatcagc cacctgagcc ccggcagaca gttcttcgag aagaagaaga ttgaccagct
     3661 catcgagaaa gagctggaac tgcacaagaa caaagtgtgc gaggaaatcc acgacaagct
     3721 gattgagctg agcctctacg actccgtgaa gaagaactgg tccaaggacg tgacaggcgc
     3781 tgccgaacag atggcctgga acagcgtgtt cctgctgccc agcctgcaga tcaccaagtt
     3841 cgtgaacgag atcgaggaac tccagcagcg gctggagaac aagggatgga agttcgaagt
     3901 gaccggcccc tggcctccct accacttcag cagctttgcc ggggcacctg gctcgggagc
     3961 gaccaacttc tcattactca aacaagccgg agacgttgag gagaatccag gccctgtgag
     4021 cctgaagcag agcatggaga ataaggatat cgccctgatc gacatcctcg acgtgatcct
     4081 ggacaaggga gtggccatca agggcgacct gatcatctct atcgccggcg tggacctggt
     4141 gtacctggat ctgagagtgc tgatctccag cgtggaaacc ctggtgcagg ccaaagaggg
     4201 caaccacaag cccatcacca gcgagcagtt cgacaagcag aaagaggagc tgatggacgc
     4261 caccggccag cccagcaagt ggacaaatcc tctgggcagc ggcgctcccg ggtcaggtgc
     4321 cacgaatttt tcgttgttga agcaagctgg ggatgttgaa gagaacccag ggcctgtgca
     4381 gcccgtgtcc caggccaacg gcagaatcca cctggatccc gatcaggccg aacagggact
     4441 ggcccagctc gtgatgaccg tgatcgagct gctgcggcag atcgtggaac ggcacgccat
     4501 gagaagagtg gaaggcggca ccctgaccga cgagcagatc gagaatctgg gaatcgctct
     4561 gatgaacctg gaggagaaga tggacgagct gaaagaggtg ttcggactgg acgctgagga
     4621 tctgaacatc gacctgggcc ctctgggcag cctgctgggt gccccgggat ctggcgcaac
     4681 aaattttagt cttttaaagc aggcaggaga cgtcgaggaa aaccctggac ccgtggccgt
     4741 ggaacacaac atgcagagca gcaccatcgt ggacgtgctg gaaaagatcc tggacaaggg
     4801 cgtcgtgatc gccggggaca tcacagtggg aatcgccgac gtggaactgc tgaccatcaa
     4861 gatccggctg atcgtggcca gcgtggacaa ggccaaagaa atcggcatgg attggtggga
     4921 gaacgacccc tacctgagca gcaagggcgc caacaacaag gctctggaag aggaaaacaa
     4981 gatgctgcac gagcggctga aaacactgga agagaagatc gagacaaagc gcggggcacc
     5041 tggctcggga gcgaccaact tctcattact caaacaagcc ggagacgttg aggagaatcc
     5101 aggccctgtg agcaccggcc ccagcttcag caccaaggac aacaccctgg aatacttcgt
     5161 gaaggccagc aacaagcacg gctttagcct cgacatcagc ctgaacgtga atggggccgt
     5221 gattagcggc accatgatca gcgccaaaga gtacttcgac tacctgagcg agacattcga
     5281 agagggcagc gaagtggccc aggccctgtc tgagcagttt agcctggcta gcgaggcctc
     5341 cgagtctaat ggcgaagccg aggcccactt catccacctg aagaacacca agatctactg
     5401 cggcgacagc aagagcaccc ccagcaaggg caagatcttc tggcgcggca agatcgccga
     5461 ggtggacgga ttcttcctgg gaaaaatcag cgacgccaag tccaccagca agaagtccag
     5521 cggcgctccc gggtcaggtg ccacgaattt ttcgttgttg aagcaagctg gggatgttga
     5581 agagaaccca gggcctgtgg tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat
     5641 cctggtggaa ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga
     5701 aggcgacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5761 cgtgccttgg cctaccctcg tgaccacact gacctacggc gtgcagtgct tcgccagata
     5821 ccccgaccac atgaagcagc acgatttctt caagagcgcc atgcccgagg gctacgtgca
     5881 ggaacggacc atcttcttca aggacgacgg caactacaag acaagagccg aagtgaagtt
     5941 cgagggcgac accctcgtga accggatcga gctgaagggc atcgacttca aagaggatgg
     6001 caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaaggtgt acatcaccgc
     6061 cgacaagcag aaaaacggca tcaaagtgaa cttcaagacc cggcacaaca tcgaggacgg
     6121 cagcgtgcag ctggccgacc actaccagca gaacaccccc atcggagatg gccccgtgct
     6181 gctgcccgac aaccactacc tgagcacaca aagcgccctg agcaaggacc ccaacgagaa
     6241 gcgggaccac atggtgctgc tggaatttgt gaccgccgct ggcatcaccc tgggcatgga
     6301 cgagctgtac aagtgaacta gttcgttaac taaacttgtt tattgcagct tataatggtt
     6361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat
     6481 gtcaattagt ctatcagaag ctcatctggt ctcccttccg ggggacaaga catccctgtt
     6541 taatatttaa acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca
     6601 ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg
     6661 tttagccccg gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg
     6721 tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc
     6781 ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg
     6841 ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg
     6901 acgtaattac atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa
     6961 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat
     7021 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg
     7081 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa
     7141 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag
     7201 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag
     7261 ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
     7321 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
     7381 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
     7441 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
     7501 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
     7561 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
     7621 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
     7681 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat
     7741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9841 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     9901 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     9961 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
    10021 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
    10081 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
    10141 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
    10201 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
    10261 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
    10321 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
    10381 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10441 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10501 ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggag
//